What's your organism?
Jon Moulton
Friday, 03 August 2007 18:52 UTC
Hi folks,
It’s been quiet on this group. Time for some posts! I’m one of the Morpholino people from Gene Tools, so while I know about my molecules I don’t have much to add regarding cool biology—I come here hoping to learn that from y’all. Who is doing what in what organism? A networking site only works if everyone is posting!
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hey everyone,
I can’t agree with Jon anymore,discussion and communication is key for the forum.Well,I’m working with Embryos of mouse and human,as my work is to establish ES cell lines and some researches related such as embryonic development.
Also,I’ve been familiar with molecular biological techniques for several years.I’d like to share my experimental experience with U,please say a word,everyone.
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Hi!
Thanks for posting an initial topic, Jon!
Shortly, I’m working on the development of a sea biscuit describing the morphology of embryos, larvae and juveniles. I’m interested in the evolution of this echinoderm group and comparative studies with marine invertebrate life histories, in general.
cheers!
ps: the avatar from DevBio group is a sea biscuit embryo.
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Alright, Humans, mice and sea biscuits. Sea biscuits? You like the mainstream models, eh Bruno? But have you eaten one ;) ?
Surely there are some zebrafish and Xenopus folks on the list—and what about the chicks, the sea urchins and the ascidians?
Seven years ago I fell into developmental biology entirely by accident. Morpholino oligos, the product of the company I work for, were fairly difficult to get into cultured cells—but they can be microinjected into embryos. Steve Ekker showed that they could phenocopy mutations in zebrafish, and suddenly I felt like I was working for a developmental bio company. I have enjoyed my on-the-job training (by reading papers and talking with the researchers who call us). But I am hoping to learn more here.
Nature networks is still young, but the discussions must start to attract more folks. How do we make this useful?
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Hi – not really up for major group animation, but I can chip in with the chick model. I also study human post-implantation embryo development.
The question as to how to make it useful – maybe we could do a small round table to post a recent article that interested you as a developmental biologist, and mention why. Not the most fabulous one you’ve ever read, just what’s on top of your reading pile currently.
For me, it was Reprogramming of human somatic cells to pluripotency with defined factors.
Ever since I was a teenager and got to see the teeth and hair in a benign cystic ovarian teratoma, I wanted to know how stem cells turn into neuroepithelial cells preferentially, and why. At the time I just wanted to know how a cell could wait to decide what it would become. So I’ve been following these “four factors” stories.
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Hello,
I work with mice and chicks. We study jaw development using lots of different molecular biology techniques.
I have a question for the mouse people out there regarding hematopoiesis. Does anyone happen to know then mature red blood cells show up in mouse? We study mice between e8.0 and e10.5. Often times cells seem to auto-fluoresce in the arteries when we do IF experiments, so I’ve been trying to figure out what they are.
Thanks for your help!
Bre -
Hi BreAnne,
A mutation in the gene encoding uroporphyrinogen decarboxylase (UROD) can turn erythrocytes fluorescent (red-emitting). Perhaps a developmental delay in expressing UROD could also trigger fluorescence.
http://www.ncbi.nlm.nih.gov/pubmed/9806541
http://www.ncbi.nlm.nih.gov/pubmed/11017081 -
BreAnne,
Is this autofluorescence or in the presence of the secondary antibodies? When doing IF on tumors I will often see the RBCs fluoresce because of crossreactivity with the secondaries.
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Hello Heather (and everyone),
Would mind starting a new topic to discuss the article you suggested, or another one on the top of your reading pile?
I think its better to discuss articles on a separate topic.
Also, I started one today, here
:)
New members, continue the thread Who is doing what in what organism?
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Hi! Thanks for your responses about the RBC’s. I’m sure the problem(background in my IF exps) is due to auto-florescence(AF) because I processed the tissue with different fix time pts, and ran them through the protocol without ANY antibody to test for AF. I found that increasing fixation, increased AF. Next, I looked at just secondaries alone and found the inverse, that is, more background from the secondary with less fix.. and the longer fixation time led to less secondary background, but more individual AFing cells, cells that I think might be RBCs. I’m looking at the tissue with the green GFP filter for AlexaFluor488 secondary. So, my question remains… when do mature erythrocytes develop in mice? It’s 4wks in humans.. so I think it might be e9.5 in mice, but I can’t seem to find any literature on it. Thank you all for your responses :-)
Also- Hi to Jon. We just ordered some Gene Tools morpholinos to disrupt neural crest cell migration in chicks. Yea!
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