What's your favorite technique?
Jonathan Singer
Friday, 16 March 2007 20:27 UTC
I’ll start the forum off with an easy question—what’s your favorite (the most fun and satisfying, not necessarily the most productive) lab technique? My two choices:
1) Titering phage: I haven’t done it in ages, but it has such an elegant linearity, how you get one clear plate, one heavily blotched one, one with 300 plaques, one with 30 and one with 3.
2) Microsatellite assays on an agarose gel: a reliable technique (once you get the assay wired) and the bands in the agarose have a physical reality to it that sequencers lack.
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Replies
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Two choices, from simplest to most complex:
1) I find pretty relaxing a protocol to extract DNA from agarose gels: the mask to protect from UVs, cutting intense-orange DNA bands from a fluorescent blue background and a series of 5-minute-centrifuge steps that allow you just relax.
2) Genotyping plattforms: it seems so high-tech and provides so huge amount information that it is very challenging!
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