Any dark side of GFP in yeast?
Chia-Yung Wu
Tuesday, 03 April 2007 16:27 EDT
I recently read a PLoS One article reporting that over-expression of GFP in higher eukaryotes may cause a number of side effects, one of which is defective polyubiquitylation. This defect depends on the peptide sequence of GFP. Has anybody noticed side effects of GFP expression in yeast?
See
http://www.plosone.org/article/fetchArticle.action?articleURI=info:doi/10.1371/journal.pone.0000054
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Replies
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Ah… Sorry. I just noticed that the link above doesn’t work.
Here it is:
The Dark Side of EGFP: Defective Polyubiquitination
Baens et. al. PLoS One Dec issue 1, 2006
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