The new image
Caption: The image shows cultured Human Umbilical Vein Endothelial Cells (HUVEC) stained for phalloidine (green), gap junction protein connexin (red) and nuclei (blue).
HUVECs is a cell type commonly used in studies related to vascular biology.
Author: Chiuhui Mary Wang
Here is a link to a related protocol.
‘A protocol for isolation and culture of human umbilical vein endothelial cells’
The new featured protocols
These describe Marc Hellerstein’s lab’s methods for mearuring cell proliferation using heavy water. Cells are grown in medium containing ^2^H~2~O (D~2~O) and the incorporation of deuterium into purine nucleotides is detected by GC/MS. Pretty cool stuff.
You can access the full text by going to:
Measurement of cell proliferation by heavy water labeling
Measurement of very low rates of cell proliferation by heavy water labeling of DNA and gas chromatography/pyrolysis/isotope ratio–mass spectrometric analysis
Some random news
Odyssey Press produces the print version of Nature Protocols and they have won a prize for “their printing quality and craftmanship in the production of Volume 2 Number 7” in the 2007 New England Regional Awards of Excellence gala.
Ask Aunty Bron
What is the difference between a Network Protocol and a Nature Protocol?
On the Nature Protocols site there are two types of content: Protocols that are peer-reviewed, edited and laid out by a production team (Nature Protocols).
Protocols that can be made available on the internet very soon after the author has uploaded them (Network Protocols).
Nature Protocols are listed in PubMed and you need a site-license to access them.
Network Protocols are freely available to everyone.
Both have DOIs and are cited as Nature Protocols.
They are listed separately on the category pages (e.g. Structural Analysis) and their appearance is alo quite different. Perhaps have a look at these two examples to see what I mean:
A Nature Protocol: ‘Purification of naturally occurring peptides by reversed-phase HPLC’
A Network Protocols on a similar subject: ‘Determination of neopterin and biopterin by liquid chromatography’
The print edition looks cool, but I’ve never seen one in person. My university subscribes to Nature Protocols and I access it online, but would love to get print copies for the lab. How does one subscribe to the print edition?
I am really pleased that you like Nature Protocols and are interested in a print copy. Here is the link that you can subscribe via:
https://secure.nature.com/subscribe/nprot
Don’t be put off by the Nature Geoscience cover — this is a (hopefully!) temporary blip.
Thanks, I thought Nature Protocols was online only. Some of us luddites still love paper.
We also thought that Nature Protocols would be on-line only! But, it turns out that we had to have at least a small print run in order to be listed in PubMed and quite a few people seem interested in print subscriptions.